The complexity of screening PMS2 in DNA isolated from formalin-fixed paraffin-embedded material
نویسندگان
چکیده
منابع مشابه
DNA extraction from formalin-fixed, paraffin-embedded tissue.
Several DNA extraction methods have been used for formalin-fixed, paraffin-embedded tissues, with variable results being reported regarding the suitability of DNA obtained from such sources to serve as template in polymerase chain reaction (PCR)-based genetic analyses. We present a method routinely used for archival material in our laboratory that reliably yields DNA of sufficient quality for P...
متن کاملUniversal amplification of DNA isolated from small regions of paraffin-embedded, formalin-fixed tissue.
The polymerase chain reaction (PCR) analysis of DNA isolated from formalin-fixed, paraffin-embedded tissue can be difficult because of the inherently poor quality of template DNA available for amplification. As a consequence of the formalin fixation process, DNA is complexed with proteins and is often nicked, giving relatively short fragments. Such samples are often of low DNA concentration and...
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Preserved clinical material is a unique source for proteomic investigation of human disorders. Here we describe an optimized protocol allowing large scale quantitative analysis of formalin fixed and paraffin embedded (FFPE) tissue. The procedure comprises four distinct steps. The first one is the preparation of sections from the FFPE material and microdissection of cells of interest. In the sec...
متن کاملNucleic Acids Extraction from Formalin-Fixed and Paraffin-Embedded Tissues
Formalin-fixed paraffin-embedded (FFPE) tissues are an important sample source for ret‐ rospective studies. Despite its ability to preserve proteins and cell morphology, formalin hinders molecular biology tests since it fragments and chemically modifies nucleic acids, especially RNA. Although several studies describe techniques that allow extracting nu‐ cleic acids from FFPE tissues, so far the...
متن کاملComparison of Methods for the Extraction of DNA from Formalin-Fixed, Paraffin-Embedded Archival Tissues
AIM Discussing a protocol involving xylene-ethanol deparaffinization on slides followed by a kit-based extraction that allows for the extraction of high quality DNA from FFPE tissues. METHODS DNA was extracted from the FFPE tissues of 16 randomly selected blocks. Methods involving deparaffinization on slides or tubes, enzyme digestion overnight or for 72 hours and isolation using phenol chlor...
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ژورنال
عنوان ژورنال: European Journal of Human Genetics
سال: 2019
ISSN: 1018-4813,1476-5438
DOI: 10.1038/s41431-019-0527-x